→ untilnow: 2 開孔位置在蛋的鈍端 你好操作就行 12/21 19:15
→ forever430: 刮出血懂意思了，剩下第一個問題跟細胞固定 12/22 00:15
→ forever430: 感謝一樓解答 12/22 00:15
→ turtle24: 吸掉一些體積讓CAM跟殼分開，就寫在同一句後面... 12/24 16:24
大家好
目前看文獻有看到將材料與CAM做貼附測試，因為不是
本科系所以不太懂這樣做的目的，實驗內容如下:
In brief, fertilized Brown Leghorn eggs (Needle Farm)
were incubated at 38C in a humidified forced flow incubator.
All procedures were carried out under the Animals Scientific
Procedures Act 1986. On day 3 of incubation, 2 ml of albumin was
removed to detach the developing chorioallantoic membrane
(CAM) from the shell; the eggs were then windowed and sealed
with adhesive tape. On day 7 of incubation, the tape was removed
and the CAM was slightly scratched with forceps to induce a small
amount of bleeding. Small pieces (approximately 2x2 mm) of
POSS-PCU(本篇使用的材料) seeded with or without hADSCs were
placed on top of the scratched area of the CAM (6 eggs per group).
The eggs werethen sealed with adhesive tape and returned to the
incubator for additional 7 days of incubation. The scaffolds were
then photographed in ovo at day 14 of incubation, before overnight
fixation in 4% PFA. The scaffolds were then washed in PBS, embedded in
OCT, sectioned and processed for immunocytochemistry as
described above.
有疑問的問題如下:
1.把2ml的蛋白去除的目的?(第四行文字)
2.開孔的位置是否有固定的地方?(上網找資料有看沒有懂orz)
3.測試之前為何要把CAM刮出血?(第八行文字)
最後還有個疑問，關於細胞固定，使用甲醇跟乙醇固定，是否有某種意義上
的差異?小弟的認知只有脫水
勞請版上鄉民指導
謝謝
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